Methods from other labs.
From Botstein Lab
Yeast, E. coli Methods from
SDS PAGE, Silver staining, Western and Northern blot, mRNA purification,
transformation, yeast colony PCR, gene deletion, and more.
- E.coli: plasmid prep and more.
General, Yeast, and
Bacteria Methods from Gottschling Lab
Southern and Northern, Oatmeal chocolate chip cookies, and more.
Plasmid rescue, Media, RNA prep, methods from Gietz lab and more.
Transformation from Gietz
Methods from this lab.
Isolation of yeast mtDNA
Primers and Method to Directly
Sequence ATP6, 8, and 9 from purified mt DNA (see
Large Scale Plasmid Preparation
Removal of primers from PCR rx for DNA sequence analysis
Beckman Conversion Tool (rpm to rcf)